pFC-MEKK

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the?in vivo?activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.

• ?Identify pathway-specific transcription activation
• ?Easier, faster than Western blotting or gel-shift assays
• ?Sensitive, quantitative assays
• ?Antibodies now available
• ?Stable cell lines available for constitutive expression of CREB, Elk1, c-Jun, and CHOP
• ?PathDetect Trans-Reporting Systems -Identify specific stimuli that leads to the phosphorylation of a test transcription factor