pGL4.18[luc2P/Neo]

The pGL4.18[luc2P/Neo] Vector(a–e)encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.18[luc2P/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with a greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

0001 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC 
0061 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT 
0121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC 
0181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC 
0241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 
0301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG 
0361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC 
0421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC 
0481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA 
0541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 
0601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC 
0661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC 
0721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC 
0781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC 
0841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC 
0901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG 
0961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC 
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG 
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC 
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC 
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC 
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT 
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC 
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC 
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC 
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG 
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA 
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC 
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG 
1741 ATCGCCGTGA ATTCTCACGG CTTCCCTCCC GAGGTGGAGG AGCAGGCCGC CGGCACCCTG 
1801 CCCATGAGCT GCGCCCAGGA GAGCGGCATG GATAGACACC CTGCTGCTTG CGCCAGCGCC 
1861 AGGATCAACG TCTAAGGCCG CGACTCTAGA GTCGGGGCGG CCGGCCGCTT CGAGCAGACA 
1921 TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT 
1981 TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC 
2041 AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG 
2101 TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCGTTTGCGT 
2161 ATTGGGCGCT CTTCCGCTGA TCTGCGCAGC ACCATGGCCT GAAATAACCT CTGAAAGAGG 
2221 AACTTGGTTA GCTACCTTCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG 
2281 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 
2341 GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT 
2401 GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC 
2461 TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA 
2521 GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG 
2581 CCTAGGCTTT TGCAAAAAGC TCGATTCTTC TGACACTAGC GCCACCATGA TCGAACAAGA 
2641 CGGCCTCCAT GCTGGCAGTC CCGCAGCTTG GGTCGAACGC TTGTTCGGGT ACGACTGGGC 
2701 CCAGCAGACC ATCGGATGTA GCGATGCGGC CGTGTTCCGT CTAAGCGCTC AAGGCCGGCC 
2761 CGTGCTGTTC GTGAAGACCG ACCTGAGCGG CGCCCTGAAC GAGCTTCAAG ACGAGGCTGC 
2821 CCGCCTGAGC TGGCTGGCCA CCACCGGCGT ACCCTGCGCC GCTGTGTTGG ATGTTGTGAC 
2881 CGAAGCCGGC CGGGACTGGC TGCTGCTGGG CGAGGTCCCT GGCCAGGATC TGCTGAGCAG 
2941 CCACCTTGCC CCCGCTGAGA AGGTTTCTAT CATGGCCGAT GCAATGCGGC GCCTGCACAC 
3001 CCTGGACCCC GCTACCTGCC CCTTCGACCA CCAGGCTAAG CATCGGATCG AGCGTGCTCG 
3061 GACCCGCATG GAGGCCGGCC TGGTGGACCA GGACGACCTG GACGAGGAGC ATCAGGGCCT 
3121 GGCCCCCGCT GAACTGTTCG CCCGACTGAA AGCCCGCATG CCGGACGGTG AGGACCTGGT 
3181 TGTCACACAC GGAGATGCCT GCCTCCCTAA CATCATGGTC GAGAATGGCC GCTTCTCCGG 
3241 CTTCATCGAC TGCGGTCGCC TAGGAGTTGC CGACCGCTAC CAGGACATCG CCCTGGCCAC 
3301 CCGCGACATC GCTGAGGAGC TTGGCGGCGA GTGGGCCGAC CGCTTCTTAG TCTTGTACGG 
3361 CATCGCAGCT CCCGACAGCC AGCGCATCGC CTTCTACCGC TTGCTCGACG AGTTCTTTTA 
3421 ATGATCTAGA ACCGGTCATG GCCGCAATAA AATATCTTTA TTTTCATTAC ATCTGTGTGT 
3481 TGGTTTTTTG TGTGTTCGAA CTAGATGCTG TCGACCGATG CCCTTGAGAG CCTTCAACCC 
3541 AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT 
3601 CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TTCCGCTTCC TCGCTCACTG 
3661 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
3721 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
3781 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
3841 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
3901 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
3961 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
4021 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
4081 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
4141 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
4201 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
4261 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
4321 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 
4381 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
4441 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
4501 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
4561 AGTAAACTTG GTCTGACAGC GGCCGCAAAT GCTAAACCAC TGCAGTGGTT ACCAGTGCTT 
4621 GATCAGTGAG GCACCGATCT CAGCGATCTG CCTATTTCGT TCGTCCATAG TGGCCTGACT 
4681 CCCCGTCGTG TAGATCACTA CGATTCGTGA GGGCTTACCA TCAGGCCCCA GCGCAGCAAT 
4741 GATGCCGCGA GAGCCGCGTT CACCGGCCCC CGATTTGTCA GCAATGAACC AGCCAGCAGG 
4801 GAGGGCCGAG CGAAGAAGTG GTCCTGCTAC TTTGTCCGCC TCCATCCAGT CTATGAGCTG 
4861 CTGTCGTGAT GCTAGAGTAA GAAGTTCGCC AGTGAGTAGT TTCCGAAGAG TTGTGGCCAT 
4921 TGCTACTGGC ATCGTGGTAT CACGCTCGTC GTTCGGTATG GCTTCGTTCA ACTCTGGTTC 
4981 CCAGCGGTCA AGCCGGGTCA CATGATCACC CATATTATGA AGAAATGCAG TCAGCTCCTT 
5041 AGGGCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCGGTG TTGTCGCTCA TGGTAATGGC 
5101 AGCACTACAC AATTCTCTTA CCGTCATGCC ATCCGTAAGA TGCTTTTCCG TGACCGGCGA 
5161 GTACTCAACC AAGTCGTTTT GTGAGTAGTG TATACGGCGA CCAAGCTGCT CTTGCCCGGC 
5221 GTCTATACGG GACAACACCG CGCCACATAG CAGTACTTTG AAAGTGCTCA TCATCGGGAA 
5281 TCGTTCTTCG GGGCGGAAAG ACTCAAGGAT CTTGCCGCTA TTGAGATCCA GTTCGATATA 
5341 GCCCACTCTT GCACCCAGTT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCGGGGTG 
5401 TGCAAAAACA GGCAAGCAAA ATGCCGCAAA GAAGGGAATG AGTGCGACAC GAAAATGTTG 
5461 GATGCTCATA CTCGTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ACTAGTACGT 
5521 CTCTCAAGGA TAAGTAAGTA ATATTAAGGT ACGGGAGGTA TTGGACAGGC CGCAATAAAA 
5581 TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG TGAATCGATA GTACTAACAT 
5641 ACGCTCTCCA TCAAAACAAA ACGAAACAAA ACAAACTAGC AAAATAGGCT GTCCCCAGTG 
5701 CAAGTGCAGG TGCCAGAACA TTTCTCT