443143416pGL4.17[luc2/Neo]
- 产品货号GS-1731
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 载体抗性Ampicillin (氨苄青霉素)
- 筛选标记Neomycin (新霉素)
- 载体大小5599 bp
- 备注说明Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay.
The pGL4.17[luc2/Neo] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.
The pGL4.17[luc2/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.
![pGL4.17[luc2/Neo]质粒图谱/载体图谱](/uploads/vector_atlas/pGL4.17_luc2_Neo_1x.png)
![pGL4.17[luc2/Neo]质粒图谱/载体图谱](/uploads/vector_atlas/pGL4.17.png)
0001 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC
0061 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGAAGATGC CAAAAACATT
0121 AAGAAGGGCC CAGCGCCATT CTACCCACTC GAAGACGGGA CCGCCGGCGA GCAGCTGCAC
0181 AAAGCCATGA AGCGCTACGC CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC
0241 GAGGTGGACA TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG
0301 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA TAGCTTGCAG
0361 TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG CTGTGGCCCC AGCTAACGAC
0421 ATCTACAACG AGCGCGAGCT GCTGAACAGC ATGGGCATCA GCCAGCCCAC CGTCGTATTC
0481 GTGAGCAAGA AAGGGCTGCA AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA
0541 AAGATCATCA TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC
0601 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA GAGCTTCGAC
0661 CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA GTACCGGATT GCCCAAGGGC
0721 GTAGCCCTAC CGCACCGCAC CGCTTGTGTC CGATTCAGTC ATGCCCGCGA CCCCATCTTC
0781 GGCAACCAGA TCATCCCCGA CACCGCTATC CTCAGCGTGG TGCCATTTCA CCACGGCTTC
0841 GGCATGTTCA CCACGCTGGG CTACTTGATC TGCGGCTTTC GGGTCGTGCT CATGTACCGC
0901 TTCGAGGAGG AGCTATTCTT GCGCAGCTTG CAAGACTATA AGATTCAATC TGCCCTGCTG
0961 GTGCCCACAC TATTTAGCTT CTTCGCTAAG AGCACTCTCA TCGACAAGTA CGACCTAAGC
1021 AACTTGCACG AGATCGCCAG CGGCGGGGCG CCGCTCAGCA AGGAGGTAGG TGAGGCCGTG
1081 GCCAAACGCT TCCACCTACC AGGCATCCGC CAGGGCTACG GCCTGACAGA AACAACCAGC
1141 GCCATTCTGA TCACCCCCGA AGGGGACGAC AAGCCTGGCG CAGTAGGCAA GGTGGTGCCC
1201 TTCTTCGAGG CTAAGGTGGT GGACTTGGAC ACCGGTAAGA CACTGGGTGT GAACCAGCGC
1261 GGCGAGCTGT GCGTCCGTGG CCCCATGATC ATGAGCGGCT ACGTTAACAA CCCCGAGGCT
1321 ACAAACGCTC TCATCGACAA GGACGGCTGG CTGCACAGCG GCGACATCGC CTACTGGGAC
1381 GAGGACGAGC ACTTCTTCAT CGTGGACCGG CTGAAGAGCC TGATCAAATA CAAGGGCTAC
1441 CAGGTAGCCC CAGCCGAACT GGAGAGCATC CTGCTGCAAC ACCCCAACAT CTTCGACGCC
1501 GGGGTCGCCG GCCTGCCCGA CGACGATGCC GGCGAGCTGC CCGCCGCAGT CGTCGTGCTG
1561 GAACACGGTA AAACCATGAC CGAGAAGGAG ATCGTGGACT ATGTGGCCAG CCAGGTTACA
1621 ACCGCCAAGA AGCTGCGCGG TGGTGTTGTG TTCGTGGACG AGGTGCCTAA AGGACTGACC
1681 GGCAAGTTGG ACGCCCGCAA GATCCGCGAG ATTCTCATTA AGGCCAAGAA GGGCGGCAAG
1741 ATCGCCGTGT AATAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA
1801 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT
1861 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
1921 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
1981 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG
2041 CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT
2101 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA
2161 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA
2221 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA
2281 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA
2341 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG
2401 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT
2461 TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT GATCGAACAA GACGGCCTCC
2521 ATGCTGGCAG TCCCGCAGCT TGGGTCGAAC GCTTGTTCGG GTACGACTGG GCCCAGCAGA
2581 CCATCGGATG TAGCGATGCG GCCGTGTTCC GTCTAAGCGC TCAAGGCCGG CCCGTGCTGT
2641 TCGTGAAGAC CGACCTGAGC GGCGCCCTGA ACGAGCTTCA AGACGAGGCT GCCCGCCTGA
2701 GCTGGCTGGC CACCACCGGC GTACCCTGCG CCGCTGTGTT GGATGTTGTG ACCGAAGCCG
2761 GCCGGGACTG GCTGCTGCTG GGCGAGGTCC CTGGCCAGGA TCTGCTGAGC AGCCACCTTG
2821 CCCCCGCTGA GAAGGTTTCT ATCATGGCCG ATGCAATGCG GCGCCTGCAC ACCCTGGACC
2881 CCGCTACCTG CCCCTTCGAC CACCAGGCTA AGCATCGGAT CGAGCGTGCT CGGACCCGCA
2941 TGGAGGCCGG CCTGGTGGAC CAGGACGACC TGGACGAGGA GCATCAGGGC CTGGCCCCCG
3001 CTGAACTGTT CGCCCGACTG AAAGCCCGCA TGCCGGACGG TGAGGACCTG GTTGTCACAC
3061 ACGGAGATGC CTGCCTCCCT AACATCATGG TCGAGAATGG CCGCTTCTCC GGCTTCATCG
3121 ACTGCGGTCG CCTAGGAGTT GCCGACCGCT ACCAGGACAT CGCCCTGGCC ACCCGCGACA
3181 TCGCTGAGGA GCTTGGCGGC GAGTGGGCCG ACCGCTTCTT AGTCTTGTAC GGCATCGCAG
3241 CTCCCGACAG CCAGCGCATC GCCTTCTACC GCTTGCTCGA CGAGTTCTTT TAATGATCTA
3301 GAACCGGTCA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT
3361 TGTGTGTTCG AACTAGATGC TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT
3421 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA
3481 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG
3541 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA
3601 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC
3661 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG
3721 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC
3781 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC
3841 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT
3901 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC
3961 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA
4021 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA
4081 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA
4141 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA
4201 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG
4261 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
4321 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
4381 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
4441 TGGTCTGACA GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG
4501 AGGCACCGAT CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG
4561 TGTAGATCAC TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA ATGATGCCGC
4621 GAGAGCCGCG TTCACCGGCC CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG
4681 AGCGAAGAAG TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG
4741 ATGCTAGAGT AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG
4801 GCATCGTGGT ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT
4861 CAAGCCGGGT CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC TTAGGGCCTC
4921 CGATCGTTGT CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC
4981 ACAATTCTCT TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA
5041 CCAAGTCGTT TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC
5101 GGGACAACAC CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT
5161 CGGGGCGGAA AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA TAGCCCACTC
5221 TTGCACCCAG TTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA
5281 CAGGCAAGCA AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA
5341 TACTCGTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG
5401 GATAAGTAAG TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA
5461 TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC
5521 CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA
5581 GGTGCCAGAA CATTTCTCT