443143416pPIC3.5
- 产品货号GS-1341
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型高
- 载体抗性Ampicillin (氨苄青霉素)
- 筛选标记HIS4
- 载体大小7751 bp
- 启动子AOX1
- 5'测序引物5? AOX1:5?-GACTGGTTCCAATTGACAAGC-3?
- 备注说明插入基因是必需包含起始密码子ATG。
pPIC3.5酵母表达载体详细信息如下:
- 载体大小为7,751 bp，是非融合蛋白表达载体。
- 单一限制性内切酶位点有BamH I, SnaB I, EcoR I, Avr II 和Not I.
- 胞内基因表达载体，不是分泌性蛋白表达载体。
- 载体构建时，需要包含一个ATG启动子，同时需要加入Kozak序列。 (Cavener and Stuart, 1991; Kozak, 1987; Kozak, 1990)
- 毕赤酵母中使用HIS4基因筛选。
- 将基因插入GS115和KM71的AOX1中，需要使用Sac I线性化质粒(GS115产生的基因型为His+ Mut+，KM71酵母产生的基因型为His+ MutS)。
- 将基因插入GS115和KM71的HIS4中，需要使用Sal I或 Stu I线性化质粒(GS115产生的基因型为His+ Mut+，KM71酵母产生的基因型为His+ MutS)。
- 将基因插入GS115的AOX1基因中，也可以使用Bgl II 限制酶线性化质粒，产生的酵母基因型为His+ MutS。
0001 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG
0061 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT
0121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC
0181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA
0241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA
0301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG
0361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT
0421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG
0481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT
0541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT
0601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT
0661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT
0721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT
0781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT
0841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA
0901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCTACGTAG AATTCCCTAG
0961 GGCGGCCGCG AATTAATTCG CCTTAGACAT GACTGTTCCT CAGTTCAAGT TGGGCACTTA
1021 CGAGAAGACC GGTCTTGCTA GATTCTAATC AAGAGGATGT CAGAATGCCA TTTGCCTGAG
1081 AGATGCAGGC TTCATTTTTG ATACTTTTTT ATTTGTAACC TATATAGTAT AGGATTTTTT
1141 TTGTCATTTT GTTTCTTCTC GTACGAGCTT GCTCCTGATC AGCCTATCTC GCAGCTGATG
1201 AATATCTTGT GGTAGGGGTT TGGGAAAATC ATTCGAGTTT GATGTTTTTC TTGGTATTTC
1261 CCACTCCTCT TCAGAGTACA GAAGATTAAG TGAGAAGTTC GTTTGTGCAA GCTTATCGAT
1321 AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG
1381 AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA
1441 GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATA TCGTCCATTC CGACAGCATC
1501 GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC
1561 GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT
1621 GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CTATCGAATC
1681 TAAATGTAAG TTAAAATCTC TAAATAATTA AATAAGTCCC AGTTTCTCCA TACGAACCTT
1741 AACAGCATTG CGGTGAGCAT CTAGACCTTC AACAGCAGCC AGATCCATCA CTGCTTGGCC
1801 AATATGTTTC AGTCCCTCAG GAGTTACGTC TTGTGAAGTG ATGAACTTCT GGAAGGTTGC
1861 AGTGTTAACT CCGCTGTATT GACGGGCATA TCCGTACGTT GGCAAAGTGT GGTTGGTACC
1921 GGAGGAGTAA TCTCCACAAC TCTCTGGAGA GTAGGCACCA ACAAACACAG ATCCAGCGTG
1981 TTGTACTTGA TCAACATAAG AAGAAGCATT CTCGATTTGC AGGATCAAGT GTTCAGGAGC
2041 GTACTGATTG GACATTTCCA AAGCCTGCTC GTAGGTTGCA ACCGATAGGG TTGTAGAGTG
2101 TGCAATACAC TTGCGTACAA TTTCAACCCT TGGCAACTGC ACAGCTTGGT TGTGAACAGC
2161 ATCTTCAATT CTGGCAAGCT CCTTGTCTGT CATATCGACA GCCAACAGAA TCACCTGGGA
2221 ATCAATACCA TGTTCAGCTT GAGACAGAAG GTCTGAGGCA ACGAAATCTG GATCAGCGTA
2281 TTTATCAGCA ATAACTAGAA CTTCAGAAGG CCCAGCAGGC ATGTCAATAC TACACAGGGC
2341 TGATGTGTCA TTTTGAACCA TCATCTTGGC AGCAGTAACG AACTGGTTTC CTGGACCAAA
2401 TATTTTGTCA CACTTAGGAA CAGTTTCTGT TCCGTAAGCC ATAGCAGCTA CTGCCTGGGC
2461 GCCTCCTGCT AGCACGATAC ACTTAGCACC AACCTTGTGG GCAACGTAGA TGACTTCTGG
2521 GGTAAGGGTA CCATCCTTCT TAGGTGGAGA TGCAAAAACA ATTTCTTTGC AACCAGCAAC
2581 TTTGGCAGGA ACACCCAGCA TCAGGGAAGT GGAAGGCAGA ATTGCGGTTC CACCAGGAAT
2641 ATAGAGGCCA ACTTTCTCAA TAGGTCTTGC AAAACGAGAG CAGACTACAC CAGGGCAAGT
2701 CTCAACTTGC AACGTCTCCG TTAGTTGAGC TTCATGGAAT TTCCTGACGT TATCTATAGA
2761 GAGATCAATG GCTCTCTTAA CGTTATCTGG CAATTGCATA AGTTCCTCTG GGAAAGGAGC
2821 TTCTAACACA GGTGTCTTCA AAGCGACTCC ATCAAACTTG GCAGTTAGTT CTAAAAGGGC
2881 TTTGTCACCA TTTTGACGAA CATTGTCGAC AATTGGTTTG ACTAATTCCA TAATCTGTTC
2941 CGTTTTCTGG ATAGGACGAC GAAGGGCATC TTCAATTTCT TGTGAGGAGG CCTTAGAAAC
3001 GTCAATTTTG CACAATTCAA TACGACCTTC AGAAGGGACT TCTTTAGGTT TGGATTCTTC
3061 TTTAGGTTGT TCCTTGGTGT ATCCTGGCTT GGCATCTCCT TTCCTTCTAG TGACCTTTAG
3121 GGACTTCATA TCCAGGTTTC TCTCCACCTC GTCCAACGTC ACACCGTACT TGGCACATCT
3181 AACTAATGCA AAATAAAATA AGTCAGCACA TTCCCAGGCT ATATCTTCCT TGGATTTAGC
3241 TTCTGCAAGT TCATCAGCTT CCTCCCTAAT TTTAGCGTTC AACAAAACTT CGTCGTCAAA
3301 TAACCGTTTG GTATAAGAAC CTTCTGGAGC ATTGCTCTTA CGATCCCACA AGGTGGCTTC
3361 CATGGCTCTA AGACCCTTTG ATTGGCCAAA ACAGGAAGTG CGTTCCAAGT GACAGAAACC
3421 AACACCTGTT TGTTCAACCA CAAATTTCAA GCAGTCTCCA TCACAATCCA ATTCGATACC
3481 CAGCAACTTT TGAGTTGCTC CAGATGTAGC ACCTTTATAC CACAAACCGT GACGACGAGA
3541 TTGGTAGACT CCAGTTTGTG TCCTTATAGC CTCCGGAATA GACTTTTTGG ACGAGTACAC
3601 CAGGCCCAAC GAGTAATTAG AAGAGTCAGC CACCAAAGTA GTGAATAGAC CATCGGGGCG
3661 GTCAGTAGTC AAAGACGCCA ACAAAATTTC ACTGACAGGG AACTTTTTGA CATCTTCAGA
3721 AAGTTCGTAT TCAGTAGTCA ATTGCCGAGC ATCAATAATG GGGATTATAC CAGAAGCAAC
3781 AGTGGAAGTC ACATCTACCA ACTTTGCGGT CTCAGAAAAA GCATAAACAG TTCTACTACC
3841 GCCATTAGTG AAACTTTTCA AATCGCCCAG TGGAGAAGAA AAAGGCACAG CGATACTAGC
3901 ATTAGCGGGC AAGGATGCAA CTTTATCAAC CAGGGTCCTA TAGATAACCC TAGCGCCTGG
3961 GATCATCCTT TGGACAACTC TTTCTGCCAA ATCTAGGTCC AAAATCACTT CATTGATACC
4021 ATTATTGTAC AACTTGAGCA AGTTGTCGAT CAGCTCCTCA AATTGGTCCT CTGTAACGGA
4081 TGACTCAACT TGCACATTAA CTTGAAGCTC AGTCGATTGA GTGAACTTGA TCAGGTTGTG
4141 CAGCTGGTCA GCAGCATAGG GAAACACGGC TTTTCCTACC AAACTCAAGG AATTATCAAA
4201 CTCTGCAACA CTTGCGTATG CAGGTAGCAA GGGAAATGTC ATACTTGAAG TCGGACAGTG
4261 AGTGTAGTCT TGAGAAATTC TGAAGCCGTA TTTTTATTAT CAGTGAGTCA GTCATCAGGA
4321 GATCCTCTAC GCCGGACGCA TCGTGGCCGG CATCACCGGC GCCACAGGTG CGGTTGCTGG
4381 CGCCTATATC GCCGACATCA CCGATGGGGA AGATCGGGCT CGCCACTTCG GGCTCATGAG
4441 CGCTTGTTTC GGCGTGGGTA TGGTGGCAGG CCCCGTGGCC GGGGGACTGT TGGGCGCCAT
4501 CTCCTTGCAT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG
4561 CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA GTATCTATGA TTGGAAGTAT
4621 GGGAATGGTG ATACCCGCAT TCTTCAGTGT CTTGAGGTCT CCTATCAGAT TATGCCCAAC
4681 TAAAGCAACC GGAGGAGGAG ATTTCATGGT AAATTTCTCT GACTTTTGGT CATCAGTAGA
4741 CTCGAACTGT GAGACTATCT CGGTTATGAC AGCAGAAATG TCCTTCTTGG AGACAGTAAA
4801 TGAAGTCCCA CCAATAAAGA AATCCTTGTT ATCAGGAACA AACTTCTTGT TTCGAACTTT
4861 TTCGGTGCCT TGAACTATAA AATGTAGAGT GGATATGTCG GGTAGGAATG GAGCGGGCAA
4921 ATGCTTACCT TCTGGACCTT CAAGAGGTAT GTAGGGTTTG TAGATACTGA TGCCAACTTC
4981 AGTGACAACG TTGCTATTTC GTTCAAACCA TTCCGAATCC AGAGAAATCA AAGTTGTTTG
5041 TCTACTATTG ATCCAAGCCA GTGCGGTCTT GAAACTGACA ATAGTGTGCT CGTGTTTTGA
5101 GGTCATCTTT GTATGAATAA ATCTAGTCTT TGATCTAAAT AATCTTGACG AGCCAAGGCG
5161 ATAAATACCC AAATCTAAAA CTCTTTTAAA ACGTTAAAAG GACAAGTATG TCTGCCTGTA
5221 TTAAACCCCA AATCAGCTCG TAGTCTGATC CTCATCAACT TGAGGGGCAC TATCTTGTTT
5281 TAGAGAAATT TGCGGAGATG CGATATCGAG AAAAAGGTAC GCTGATTTTA AACGTGAAAT
5341 TTATCTCAAG ATCTCTGCCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG
5401 CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT
5461 CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCGCAG CCATGACCCA GTCACGTAGC
5521 GATAGCGGAG TGTATACTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
5581 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT
5641 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
5701 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
5761 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
5821 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
5881 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
5941 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
6001 GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
6061 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
6121 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
6181 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
6241 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
6301 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
6361 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
6421 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
6481 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
6541 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
6601 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
6661 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
6721 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
6781 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
6841 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG
6901 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
6961 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
7021 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
7081 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
7141 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC
7201 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
7261 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
7321 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
7381 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
7441 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
7501 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
7561 AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC
7621 GTATCACGAG GCCCTTTCGT CTTCAAGAAT TAATTCTCAT GTTTGACAGC TTATCATCGA
7681 TAAGCTGACT CATGTTGGTA TTGTGAAATA GACGCAGATC GGGAACACTG AAAAATAACA
7741 GTTATTATTC G