443143416pGAPZ A
- 产品货号GS-1991
- 销售价格￥ 816
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型高
- 载体抗性Zeocin (博莱霉素)
- 载体大小2884 bp
- 启动子GAP
- 5'测序引物pGAP-F:5?-GTCCCTATTTCAATCAATTGAA-3?
- 备注说明插入基因是必需包含起始密码子ATG。
GAP启动子（PGAP）的重组蛋白表达水平略高于AOX1启动子。 pGAPZ A，B，和C载体（2.9 KB）和pGAPZα，B，和C（3.1 KB）载体使用GAP启动子在毕赤酵母中稳定表达重组蛋白。重组蛋白表达为为带有C-末端myc抗原表位和C端His标签的融合蛋白。此外，pGAPZα表达的融合蛋白在N-端带有酿酒酵母（Saccharomyces cerevisiae）的α-因子分泌信号肽。这两个系列的载体都提供三个不同读码框的载体，以方便克隆的带有C-端标签和/或N-末端分泌信号肽的载体中。 这些载体是基于显性选择标记，Zeocin（博莱霉素）的筛选标记可以同时使用于毕赤酵母和大肠杆菌。
0001 AGATCTTTTT TGTAGAAATG TCTTGGTGTC CTCGTCCAAT CAGGTAGCCA TCTCTGAAAT
0061 ATCTGGCTCC GTTGCAACTC CGAACGACCT GCTGGCAACG TAAAATTCTC CGGGGTAAAA
0121 CTTAAATGTG GAGTAATGGA ACCAGAAACG TCTCTTCCCT TCTCTCTCCT TCCACCGCCC
0181 GTTACCGTCC CTAGGAAATT TTACTCTGCT GGAGAGCTTC TTCTACGGCC CCCTTGCAGC
0241 AATGCTCTTC CCAGCATTAC GTTGCGGGTA AAACGGAGGT CGTGTACCCG ACCTAGCAGC
0301 CCAGGGATGG AAAAGTCCCG GCCGTCGCTG GCAATAATAG CGGGCGGACG CATGTCATGA
0361 GATTATTGGA AACCACCAGA ATCGAATATA AAAGGCGAAC ACCTTTCCCA ATTTTGGTTT
0421 CTCCTGACCC AAAGACTTTA AATTTAATTT ATTTGTCCCT ATTTCAATCA ATTGAACAAC
0481 TATTTCGAAA CGAGGAATTC ACGTGGCCCA GCCGGCCGTC TCGGATCGGT ACCTCGAGCC
0541 GCGGCGGCCG CCAGCTTGGG CCCGAACAAA AACTCATCTC AGAAGAGGAT CTGAATAGCG
0601 CCGTCGACCA TCATCATCAT CATCATTGAG TTTTAGCCTT AGACATGACT GTTCCTCAGT
0661 TCAAGTTGGG CACTTACGAG AAGACCGGTC TTGCTAGATT CTAATCAAGA GGATGTCAGA
0721 ATGCCATTTG CCTGAGAGAT GCAGGCTTCA TTTTTGATAC TTTTTTATTT GTAACCTATA
0781 TAGTATAGGA TTTTTTTTGT CATTTTGTTT CTTCTCGTAC GAGCTTGCTC CTGATCAGCC
0841 TATCTCGCAG CTGATGAATA TCTTGTGGTA GGGGTTTGGG AAAATCATTC GAGTTTGATG
0901 TTTTTCTTGG TATTTCCCAC TCCTCTTCAG AGTACAGAAG ATTAAGTGAG ACCTTCGTTT
0961 GTGCGGATCC CCCACACACC ATAGCTTCAA AATGTTTCTA CTCCTTTTTT ACTCTTCCAG
1021 ATTTTCTCGG ACTCCGCGCA TCGCCGTACC ACTTCAAAAC ACCCAAGCAC AGCATACTAA
1081 ATTTTCCCTC TTTCTTCCTC TAGGGTGTCG TTAATTACCC GTACTAAAGG TTTGGAAAAG
1141 AAAAAAGAGA CCGCCTCGTT TCTTTTTCTT CGTCGAAAAA GGCAATAAAA ATTTTTATCA
1201 CGTTTCTTTT TCTTGAAATT TTTTTTTTTA GTTTTTTTCT CTTTCAGTGA CCTCCATTGA
1261 TATTTAAGTT AATAAACGGT CTTCAATTTC TCAAGTTTCA GTTTCATTTT TCTTGTTCTA
1321 TTACAACTTT TTTTACTTCT TGTTCATTAG AAAGAAAGCA TAGCAATCTA ATCTAAGGGC
1381 GGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG
1441 AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC
1501 GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC
1561 TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG
1621 GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG
1681 TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC
1741 GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC
1801 TTCGTGGCCG AGGAGCAGGA CTGACACGTC CGACGGCGGC CCACGGGTCC CAGGCCTCGG
1861 AGATCCGTCC CCCTTTTCCT TTGTCGATAT CATGTAATTA GTTATGTCAC GCTTACATTC
1921 ACGCCCTCCC CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA
1981 GGTCCCTATT TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT
2041 TTTTCTTTTT TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT
2101 TGAGAAGGTT TTGGGACGCT CGAAGGCTTT AATTTGCAAG CTGGAGACCA ACATGTGAGC
2161 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
2221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
2281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
2341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
2401 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
2461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
2521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
2581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
2641 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
2701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
2761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
2821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCATGA
2881 GATC