443143416pAc5.1/V5-His C
- 产品货号GS-1089
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 载体抗性Ampicillin (氨苄青霉素)
- 载体大小5371 bp
- 启动子pAC5
- 5'测序引物AC5:5'd[ACACAAAGCCGCTCCATCAG]3'
- 备注说明Constitutive insect cell expression. Can use pCoHygro or pCoBlast for selection.
The DES?-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the?Drosophila?actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
- Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
0001 CTTCAAGGAA TTAATTCTAT ATTCTAAAAA CACAAATGAT ACTTCTAAAA AAAATCATGA
0061 ATGGCATCAA CTCTGAATCA AATCTTTGCA GATGCACCTA CTTCTCATTT CCACTGTCAC
0121 ATCATTTTTC CAGATCTCGC TGCCTGTTAT GTGGCCCACA AACCAAGACA CGTTTTATGG
0181 CCATTAAAGC TGGCTGATCG TCGCCAAACA CCAAATACAT ATCAATATGT ACATTCGAGA
0241 AAGAAGCGAT CAAAGAAGCG TCTTCGGGCG AGTAGGAGAA TGCGGAGGAG AAGGAGAACG
0301 AGCTGATCTA GTATCTCTCC ACAATCCAAT GCCAACTGAC CAACTGGCCA TATTCGGAGC
0361 AATTTGAAGC CAATTTCCAT CGCCTGGCGA TCGCTCCATT CTTGGCTATA TGTTTTTCAC
0421 CGTTCCCGGG GCCATTTTCA AAGACTCGTC GGTAAGATAA GATTGTGTCA CTCGCTGTCT
0481 CTCTTCATTT GTCGAAGAAT GCTGAGGAAT TTCGCGATGA CGTCGGCGAG TATTTTGAAG
0541 AATGAGAATA ATTTGTATTT ATACGAAAAT CAGTTAGTGG AATTTTCTAC AAAAACATGT
0601 TATCTATAGA TAATTTTGTT GCAAAATATG TTGACTATGA CAAAGATTGT ATGTATATAC
0661 CTTTAATGTA TTCTCATTTT CTTATGTATT TATAATGGCA ATGATGATAC TGATGATATT
0721 TTAAGATGAT GCCAGACCAC AGGCTGATTT CTGCGTCTTT TGCCGAACGC AGTGCATGTG
0781 CGGTTGTTGT TTTTTGGAAT AGTTTCAATT TTCGGACTGT CCGCTTTGAT TTCAGTTTCT
0841 TGGCTTATTC AAAAAGCAAA GTAAAGCCAA AAAAGCGAGA TGGCAATACC AAATGCGGCA
0901 AAACGGTAGT GGAAGGAAAG GGGTGCGGGG CAGCGGAAGG AAGGGTGGGG CGGGGCGTGG
0961 CGGGGTCTGT GGCTGGGCGC GACGTCACCG ACGTTGGAGC CACTCCTTTG ACCATGTGTG
1021 CGTGTGTGTA TTATTCGTGT CTCGCCACTC GCCGGTTGTT TTTTTCTTTT TATCTCGCTC
1081 TCTCTAGCGC CATCTCGTAC GCATGCTCAA CGCACCGCAT GTTGCCGTGT CCTTTATGCG
1141 TCATTTTGGC TCGAAATAGG CAATTATTTA AACAAAGATT AGTCAACGAA AACGCTAAAA
1201 TAAATAAGTC TACAATATGG TTACTTATTG CCATGTGTGT GCAGCCAACG ATAGCAACAA
1261 AAGCAACAAC ACAGTGGCTT TCCCTCTTTC ACTTTTTGTT TGCAAGCGCG TGCGAGCAAG
1321 ACGGCACGAC CGGCAAACGC AATTACGCTG ACAAAGAGCA GACGAAGTTT TGGCCGAAAA
1381 ACATCAAGGC GCCTGATACG AATGCATTTG CAATAACAAT TGCGATATTT AATATTGTTT
1441 ATGAAGCTGT TTGACTTCAA AACACACAAA AAAAAAAATA AAACAAATTA TTTGAAAGAG
1501 AATTAGGAAT CGGACAGCTT ATCGTTACGG GCTAACAGCA CACCGAGACG AAATAGCTTA
1561 CCTGACGTCA CAGCCTCTGG AAGAACTGCC GCCAAGCAGA GAGAGAGAGA AAAAGAGGGA
1621 GAGCAGCTTA GACCGCATGT GCTTGTGTGT GAGGCGTCTC TCTCTTCGTC TCCTGTTTGC
1681 GCAAACGCAT AGACTGCACT GAGAAAATCG ATTACCTATT TTTTATGAAT GAATATTTGC
1741 ACTATTACTA TTCAAAACTA TTAAGATAGC AATCACATTC AATAGCCAAA TACTATACCA
1801 CCTGAGCGAT GCAACGAAAT GATCAATTTG AGCAAAAATG CTGCATATTT AGGACGGCAT
1861 CATTATAGAA ATGCTTCTTG CTGTGTACTT TTCTCTCGTC TGGCAGCTGT TTCGCCGTTA
1921 TTGTTAAAAC CGGCTTAAGT TAGGTGTGTT TTCTACGACT AGTGATGCCC CTACTAGAAG
1981 ATGTGTGTTG CACAAATGTC CCTGAATAAC CAATTTGAAG TGCAGATAGC AGTAAACGTA
2041 AGCTAATATG AATATTATTT AACTGTAATG TTTTAATATC GCTGGACATT ACTAATAAAC
2101 CCACTATAAA CACATGTACA TATGTATGTT TTGGCATACA ATGAGTAGTT GGGGAAAAAA
2161 TGTGTAAAAG CACCGTGACC ATCACAGCAT AAAGATAACC AGCTGAAGTA TCGAATATGA
2221 GTAACCCCCA AATTGAATCA CATGCCGCAA CTGATAGGAC CCATGGAAGT ACACTCTTCA
2281 TGGCGATATA CAAGACACAC ACAAGCACGA ACACCCAGTT GCGGAGGAAA TTCTCCGTAA
2341 ATGAAAACCC AATCGGCGAA CAATTCATAC CCATATATGG TAAAAGTTTT GAACGCGACT
2401 TGAGAGCGGA GAGCATTGCG GCTGATAAGG TTTTAGCGCT AAGCGGGCTT TATAAAACGG
2461 GCTGCGGGAC CAGTTTTCAT ATCACTACCG TTTGAGTTCT TGTGCTGTGT GGATACTCCT
2521 CCCGACACAA AGCCGCTCCA TCAGCCAGCA GTCGTCTAAT CCAGAGACCC CGGATCGGGG
2581 TACCTACTAG TCCAGTGTGG TGGAATTCTG CAGATATCCA GCACAGTGGC GGCCGCTCGA
2641 GGTCACCCAT TCGAAGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGCGT
2701 ACCGGTCATC ATCACCATCA CCATTGAGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT
2761 TCTAAGGCCT GAGCTCGCTG ATCAGCCTCG ATCGAGGATC CAGACATGAT AAGATACATT
2821 GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT
2881 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC
2941 AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
3001 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT CGACCGATGC CCTTGAGAGC
3061 CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT
3121 GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT
3181 CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG
3241 AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA
3301 GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT TGCTGGCGTT
3361 CGCGACGCGA GGCTGGATGG CCTTCCCCAT TATGATTCTT CTCGCTTCCG GCGGCATCGG
3421 GATGCCCGCG TTGCAGGCCA TGCTGTCCAG GCAGGTAGAT GACGACCATC AGGGACAGCT
3481 TCAAGGATCG CTCGCGGCTC TTACCAGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
3541 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
3601 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
3661 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
3721 CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
3781 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
3841 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
3901 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
3961 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT
4021 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
4081 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
4141 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
4201 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
4261 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
4321 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
4381 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
4441 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
4501 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
4561 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
4621 CATTGCTGCA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
4681 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
4741 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
4801 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
4861 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
4921 GGCGTCAACA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
4981 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
5041 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
5101 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
5161 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
5221 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
5281 ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA
5341 TAAAAATAGG CGTATCACGA GGCCCTTTCG T