443143416pcDNA3.1/Zeo(+)
- 产品货号GS-1012
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型高
- 载体抗性Ampicillin (氨苄青霉素)
- 筛选标记Zeocin 博来霉素
- 载体大小5015 bp
- 启动子CMV
- 5'测序引物pcDNA3.1-F:CTAGAGAACCCACTGCTTAC
- 3'测序引物pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG
- 备注说明Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.
This pcDNA?3.1/Zeo(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Zeocin? selectable marker and a forward-orientation multiple cloning site.
The pcDNA?3.1 Expression Vector Family
Three untagged versions of pcDNA?3.1 (available separately), each with a different selectable marker (Geneticin?, Zeocin?, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
- Cytomegalovirus (CMV) enhancer-promoter for high-level expression
- Large multiple cloning site in either forward (+) or reverse (-) orientations
- Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
- Ampicillin resistance gene and pUC origin for selection and maintenance in?E. coli
