443143416pTRE2hyg
- 产品货号GS-1723
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 载体抗性Ampicillin (氨苄青霉素)
- 筛选标记Hygromycin (潮霉素)
- 载体大小5325 bp
pTRE2hyg is a response plasmid that expresses a gene of interest (Gene X) in CLONTECH's Tet-On? and Tet-Off? Gene Expression Systems and Tet-On and Tet-Off Cell Lines (1). The Tet Expression Systems and Cell Lines give researchers ready access to the tetracycline-regulated expression systems described by Gossen & Bujard (2; Tet-Off) and Gossen?et al. (3; Tet-On). pTRE2hyg contains an MCS immediately downstream of the Tet-responsive?PhCMV*-1?promoter. cDNAs or genes inserted into the MCS will be responsive to the tTA and rtTA regulatory proteins in the Tet-Off and Tet-On systems, respectively.?PhCMV*-1?contains the Tet response element (TRE), which consists of seven copies of the 19-bp tet operator sequence (tetO). The TRE element is just upstream of the minimal CMV promoter (Pmin CMV), which lacks the enhancer that is part of the complete CMV promoter. Consequently,?PhCMV*-1?is silent in the absence of binding of TetR or rTetR to the?tetO?sequences. Note that the cloned insert must have an initiating ATG codon. In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE2hyg also contains the hygromycin resistance gene for direct selection of stable transformants. The parental vector pTRE2 was originally described as pUHD10-3 in reference 5.
The pTRE2hyg-Luc Control Vector, packaged with the pTRE2hyg Vector, contains an additional 1649 bp encoding firefly luciferase inserted into the MCS. This vector can be used as a reporter of induction efficiency using standard luciferase detection reagents. It is not intended as a cloning vector.
0001 CTCGAGTTTA CCACTCCCTA TCAGTGATAG AGAAAAGTGA AAGTCGAGTT TACCACTCCC
0061 TATCAGTGAT AGAGAAAAGT GAAAGTCGAG TTTACCACTC CCTATCAGTG ATAGAGAAAA
0121 GTGAAAGTCG AGTTTACCAC TCCCTATCAG TGATAGAGAA AAGTGAAAGT CGAGTTTACC
0181 ACTCCCTATC AGTGATAGAG AAAAGTGAAA GTCGAGTTTA CCACTCCCTA TCAGTGATAG
0241 AGAAAAGTGA AAGTCGAGTT TACCACTCCC TATCAGTGAT AGAGAAAAGT GAAAGTCGAG
0301 CTCGGTACCC GGGTCGAGGT AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT
0361 TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC
0421 ACCGGGACCG ATCCAGCCTC CGCGGCCCCG AATTCGAGCT CGGTACCCGG GGATCCTCTA
0481 GTCAGCTGAC GCGTGCTAGC GCGGCCGCAT CGATAAGCTT GTCGACGATA TCTCTAGAGC
0541 TGAGAACTTC AGGGTGAGTT TGGGGACCCT TGATTGTTCT TTCTTTTTCG CTATTGTAAA
0601 ATTCATGTTA TATGGAGGGG GCAAAGTTTT CAGGGTGTTG TTTAGAATGG GAAGATGTCC
0661 CTTGTATCAC CATGGACCCT CATGATAATT TTGTTTCTTT CACTTTCTAC TCTGTTGACA
0721 ACCATTGTCT CCTCTTATTT TCTTTTCATT TTCTGTAACT TTTTTCGTTA AACTTTAGCT
0781 TGCATTTGTA ACGAATTTTT AAATTCACTT TCGTTTATTT GTCAGATTGT AAGTACTTTC
0841 TCTAATCACT TTTTTTTCAA GGCAATCAGG GTAATTATAT TGTACTTCAG CACAGTTTTA
0901 GAGAACAATT GTTATAATTA AATGATAAGG TAGAATATTT CTGCATATAA ATTCTGGCTG
0961 GCGTGGAAAT ATTCTTATTG GTAGAAACAA CTACATCCTG GTAATCATCC TGCCTTTCTC
1021 TTTATGGTTA CAATGATATA CACTGTTTGA GATGAGGATA AAATACTCTG AGTCCAAACC
1081 GGGCCCCTCT GCTAACCATG TTCATGCCTT CTTCTTTTTC CTACAGCTCC TGGGCAACGT
1141 GCTGGTTGTT GTGCTGTCTC ATCATTTTGG CAAAGAATTC ACTCCTCAGG TGCAGGCTGC
1201 CTATCAGAAG GTGGTGGCTG GTGTGGCCAA TGCCCTGGCT CACAAATACC ACTGAGATCT
1261 TTTTCCCTCT GCCAAAAATT ATGGGGACAT CATGAAGCCC CTTGAGCATC TGACTTCTGG
1321 GTAATAAAGG AAATTTATTT TCATTGCAAT AGTGTGTGGG AATTTTTTGT GTCTCTCACT
1381 CGGAAGGACA TATGGGAGGG CAAATCATTT AAAACATCAG AATGAGTATT TGGTTTAGAG
1441 TTTGGCAACA TATGCCATAT GCTGGCTGCC ATGAACAAAG GTGGCTATAA AGAGGTCATC
1501 AGTATATGAA ACAGCCCCCT GCTGTCCATT CCTTATTCCA TAGAAAAGCC TTGACTTGAG
1561 GTTAGATTTT TTTTATATTT TGTTTTGTGT TATTTTTTTC TTTAACATCC CTAAAATTTT
1621 CCTTACATGT TTTACTAGCC AGATTTTTCC TCCTCTCCTG ACTACTCCCA GTCATAGCTG
1681 TCCCTCTTCT CTTATGAACT CGACTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
1741 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
1801 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
1861 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
1921 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
1981 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
2041 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
2101 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT
2161 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
2221 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
2281 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
2341 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
2401 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
2461 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
2521 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
2581 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
2641 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
2701 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
2761 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
2821 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
2881 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
2941 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
3001 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
3061 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
3121 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
3181 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
3241 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
3301 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
3361 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
3421 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
3481 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
3541 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
3601 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
3661 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
3721 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGAGGCAGTG AAAAAAATGC
3781 TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA
3841 CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG
3901 GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG CTGATTATGA TCCTCTAGAA
3961 CTCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC GGCGAGTACT
4021 TCTACACAGC CATCGGTCCA GACGGCCGCG CTTCTGCGGG CGATTTGTGT ACGCCCGACA
4081 GTCCCGGCTC CGGATCGGAC GATTGCGTCG CATCGACCCT GCGCCCAAGC TGCATCATCG
4141 AAATTGCCGT CAACCAAGCT CTGATAGAGT TGGTCAAGAC CAATGCGGAG CATATACGCC
4201 CGGAGCCGCG GCGATCCTGC AAGCTCCGGA TGCCTCCGCT CGAAGTAGCG CGTCTGCTGC
4261 TCCATACAAG CCAACCACGG CCTCCAGAAG AAGATGTTGG CGACCTCGTA TTGGGAATCC
4321 CCGAACATCG CCTCGCTCCA GTCAATGACC GCTGTTATGC GGCCATTGTC CGTCAGGACA
4381 TTGTTGGAGC CGAAATCCGC GTGCACGAGG TGCCGGACTT CGGGGCAGTC CTCGGCCCAA
4441 AGCATCAGCT CATCGAGAGC CTGCGCGACG GACGCACTGA CGGTGTCGTC CATCACAGTT
4501 TGCCAGTGAT ACACATGGGG ATCAGCAATC GCGCATATGA AATCACGCCA TGTAGTGTAT
4561 TGACCGATTC CTTGCGGTCC GAATGGGCCG AACCCGCTCG TCTGGCTAAG ATCGGCCGCA
4621 GCGATCGCAT CCATGGCCTC CGCGACCGGC TGCAGAACAG CGGGCAGTTC GGTTTCAGGC
4681 AGGTCTTGCA ACGTGACACC CTGTGCACGG CGGGAGATGC AATAGGTCAG GCTCTCGCTG
4741 AATTCCCCAA TGTCAAGCAC TTCCGGAATC GGGAGCGCGG CCGATGCAAA GTGCCGATAA
4801 ACATAACGAT CTTTGTAGAA ACCATCGGCG CAGCTATTTA CCCGCAGGAC ATATCCACGC
4861 CCTCCTACAT CGAAGCTGAA AGCACGAGAT TCTTCGCCCT CCGAGAGCTG CATCAGGTCG
4921 GAGACGCTGT CGAACTTTTC GATCAGAAAC TTCTCGACAG ACGTCGCGGT GAGTTCAGGC
4981 TTTTTCATGG AAGCTTTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT
5041 GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC
5101 CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG
5161 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG
5221 AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC
5281 TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACA