443143416pLVTH
- 产品货号GS-1411
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型高
- 载体抗性Ampicillin (氨苄青霉素)
- 载体大小11086 bp
- 启动子EF-1 alpha
- 5'测序引物EF-1a Forward: TCAAGCCTCAGACAGTGGTTC
- 备注说明载体使用Stbl3感受态细胞扩增质粒，可以减少载体基因重组几率。该载体已经被pLVTHM载体替代，pLVTHM载体允许shRNA的定向克隆。
载体说明
This vector has been replaced by the newer pLVTHM vector, which allows for direct cloning of shRNA. For pLVTH: if you have your shRNA already in pSUPER (or any other plasmid under control of PolIII promoter) you may use EcoR1-Cla1 sites to replace H1 promoter in pLVTH with H1-shRNA cassette from pSUPER (or other plasmid). Please note that there is an additional ClaI site in this vector that is blocked by Dam methylation. This plasmid needs to be grown in a Dam+ bacteria strain if you wish to use ClaI for cloning. Article: Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. Wiznerowicz M et al. (J Virol. 2003 Aug . 77(16):8957-61.
