443143416pIZT/V5-His
- 产品货号GS-1178
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型高
- 载体抗性Zeocin (博莱霉素)
- 筛选标记Zeocin
- 载体大小3336 bp
- 启动子OpIE2
- 5'测序引物OpIE2：5'd[CGCAACGATCTGGTAAACAC]3'
- 备注说明Stable expression of gene in insect cells. GFP reporter allows visualization of stable cells.
载体描述
The InsectSelect? Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
- The OpIE2 promoter for constitutive expression
- The Zeocin? resistance gene for rapid selection of stably transfected cell lines
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin? resistanceprotein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lineswith Zeocin? and confers a fluorescent phenotype that simplifies identification of transfected cells.
