443143416pBad/gIII B
- 产品货号GS-1926
- 销售价格￥ 1020
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型高
- 载体抗性Ampicillin (氨苄青霉素)
- 载体大小4147 bp
- 启动子araBad
- 5'测序引物pBAD-F: ATGCCATAGCATTTTTATCC
pBAD/gIII载体质粒是衍生于pBR322载体。载体设计用来在大肠杆菌中进行可调节分泌表达和纯化重组目的蛋白。使用基因III信号序列来定位重组蛋白，使其分泌到大肠杆菌周质腔中。使用大肠杆菌araBAD启动子（pBAD）增强了大肠杆菌重组蛋白可溶性表达的水平。载体上的调节蛋白AraC能够调控pBad启动子。
0001 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
0061 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
0121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
0181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
0241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC
0301 TAACAGGAGG AATTAACCAT GAAAAAACTG CTGTTCGCGA TTCCGCTGGT GGTGCCGTTC
0361 TATAGCCATA GCACCATGGC TAGCTCGAGA TCTGCAGCTG GTACCATATG GGAATTCGAA
0421 GCTTTCTAGA ACAAAAACTC ATCTCAGAAG AGGATCTGAA TAGCGCCGTC GACCATCATC
0481 ATCATCATCA TTGAGTTTAA ACGGTCTCCA GCTTGGCTGT TTTGGCGGAT GAGAGAAGAT
0541 TTTCAGCCTG ATACAGATTA AATCAGAACG CAGAAGCGGT CTGATAAAAC AGAATTTGCC
0601 TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG AACTCAGAAG TGAAACGCCG
0661 TAGCGCCGAT GGTAGTGTGG GGTCTCCCCA TGCGAGAGTA GGGAACTGCC AGGCATCAAA
0721 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
0781 ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC
0841 CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GCATCAAATT AAGCAGAAGG
0901 CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT TTTTGTTTAT TTTTCTAAAT
0961 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
1021 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
1081 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
1141 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
1201 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
1261 CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
1321 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
1381 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
1441 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
1501 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
1561 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
1621 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
1681 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
1741 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
1801 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
1861 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
1921 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
1981 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
2041 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
2101 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
2161 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT
2221 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
2281 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
2341 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
2401 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
2461 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
2521 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
2581 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
2641 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
2701 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
2761 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
2821 CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
2881 ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA
2941 TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC
3001 GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC
3061 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG
3121 CAGATCAATT CGCGCGCGAA GGCGAAGCGG CATGCATAAT GTGCCTGTCA AATGGACGAA
3181 GCAGGGATTC TGCAAACCCT ATGCTACTCC GTCAAGCCGT CAATTGTCTG ATTCGTTACC
3241 AATTATGACA ACTTGACGGC TACATCATTC ACTTTTTCTT CACAACCGGC ACGGAACTCG
3301 CTCGGGCTGG CCCCGGTGCA TTTTTTAAAT ACCCGCGAGA AATAGAGTTG ATCGTCAAAA
3361 CCAACATTGC GACCGACGGT GGCGATAGGC ATCCGGGTGG TGCTCAAAAG CAGCTTCGCC
3421 TGGCTGATAC GTTGGTCCTC GCGCCAGCTT AAGACGCTAA TCCCTAACTG CTGGCGGAAA
3481 AGATGTGACA GACGCGACGG CGACAAGCAA ACATGCTGTG CGACGCTGGC GATATCAAAA
3541 TTGCTGTCTG CCAGGTGATC GCTGATGTAC TGACAAGCCT CGCGTACCCG ATTATCCATC
3601 GGTGGATGGA GCGACTCGTT AATCGCTTCC ATGCGCCGCA GTAACAATTG CTCAAGCAGA
3661 TTTATCGCCA GCAGCTCCGA ATAGCGCCCT TCCCCTTGCC CGGCGTTAAT GATTTGCCCA
3721 AACAGGTCGC TGAAATGCGG CTGGTGCGCT TCATCCGGGC GAAAGAACCC CGTATTGGCA
3781 AATATTGACG GCCAGTTAAG CCATTCATGC CAGTAGGCGC GCGGACGAAA GTAAACCCAC
3841 TGGTGATACC ATTCGCGAGC CTCCGGATGA CGACCGTAGT GATGAATCTC TCCTGGCGGG
3901 AACAGCAAAA TATCACCCGG TCGGCAAACA AATTCTCGTC CCTGATTTTT CACCACCCCC
3961 TGACCGCGAA TGGTGAGATT GAGAATATAA CCTTTCATTC CCAGCGGTCG GTCGATAAAA
4021 AAATCGAGAT AACCGTTGGC CTCAATCGGC GTTAAACCCG CCACCAGATG GGCATTAAAC
4081 GAGTATCCCG GCAGCAGGGG ATCATTTTGC GCTTCAGCCA TACTTTTCAT ACTCCCGCCA
4141 TTCAGAG