443143416pVE5523
- 产品货号GS-9014
- 销售价格￥ 0
- 包装规格2μg质粒或0.5ml甘油菌
- 克隆方法Sal I和Eco RI，限制性内切酶
- 载体抗性氨卞青霉素
- 筛选标记红霉素
- 载体大小8328 bp
- 启动子P59
穿梭载体，可以在大肠杆菌中复制，在乳酸菌表达蛋白。
0001 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
0061 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
0121 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
0181 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
0241 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
0301 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
0361 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
0421 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
0481 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
0541 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
0601 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
0661 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
0721 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
0781 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
0841 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
0901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
0961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
1021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
1081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
1141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
1201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
1261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
1321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
1381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
1441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
1501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
1561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
1621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
1681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
1741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
1801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
1861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
1921 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
1981 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
2041 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
2101 ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa
2161 aagctggtac cgggcccccc ctcgacggta tcgatagccc gcctaatgag cgggcttttt
2221 tttgatcccc taaccaaaga agcgcgtaat atcagggttt aagagacaat aagaaaaaaa
2281 gattgaaaaa gtgacattaa attcttgaca gggagagata ggtttgatag aatataatag
2341 gggtaccgag ctcgaattcc tgcagcccaa atcgcgaaac cgaacttaat gggaggaaaa
2401 attaaaaaag aacagttatg aaaaaaaaga ttatctcagc tattttaatg tctacagtga
2461 tactttctgc tgcagccccg ttgtcaggtg tttacgctga cacaaactca gatattgcta
2521 aacaagatgc ggtcgaccca ttgtcaacta aaaaattaca taaagaacct gcgactttaa
2581 ttaaagcgat tgatggtgat acggttaaat taatgtacaa aggtcaacca atgacattca
2641 gactattatt ggttgataca cctgaaacaa agcatcctaa aaaaggtgta gagaaatatg
2701 gtcctgaagc aagtgcattt acgaaaaaaa tggtagaaaa tgcaaagaaa attgaagtcg
2761 agtttgacaa aggtcaaaga actgataaat atggacgtgg cttagcgtat atttatgctg
2821 atggaaaaat ggtaaacgaa gctttagttc gtcaaggctt ggctaaagtt gcttatgttt
2881 acaaacctaa caatacacat gaacaacatt taagaaaaag tgaagcacaa gcgaaaaaag
2941 agaaattaaa tatttggagc gaagacaacg ctgattcagg tcaaatcgat ctcgagatat
3001 cgctagttct agattgaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
3061 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgctcta
3121 gctaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttgttggg
3181 gatccgcctg cagccaagct tatcgattct agactcgaga gctctcgagt ctagaatcga
3241 tacgattttg aagtggcaac agataaaaaa aagcagttta aaattgttgc tgaactttta
3301 aaacaagcaa atacaatcat tgtcgcaaca gatagcgaca gagaaggcga aaacattgcc
3361 tggtcgatca ttcataaagc aaatgccttt tctaaagata aaacgtataa aagactatgg
3421 atcaatagtt tagaaaaaga tgtgatccgt agcggttttc aaaatttgca accaggaatg
3481 aattactatc ccttttatca agaagcgcac aaaaagaaaa acgaaatgat acaccaatca
3541 gtgcaaaaaa agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata
3601 tcataaaaat cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gaaataagac
3661 ttagaagcaa acttaagagt gtgttgatag tgcagtatct taaaattttg tataatagga
3721 attgaagtta aattagatgc taaaaatttg taattaagaa ggagtgatta catgaacaaa
3781 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
3841 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
3901 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
3961 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
4021 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggag tattccttac
4081 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
4141 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
4201 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
4261 cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
4321 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
4381 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
4441 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
4501 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
4561 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctagc
4621 gcttagaatc gctttaggaa acacgatcca gtccaataat cgtcgataaa aacttttgaa
4681 aaaggttggt gaaattacct acttttggaa taatcacaaa tcacaagtga ttaatcacaa
4741 atcacaagtg attaatcact tgtttattaa gatattaaaa gctataattt aaataaagcg
4801 tgaattttat tacacaaaaa gaggggggag aaacttggaa ctagcattta gagaaagctt
4861 aaaaaagatg agaggtacca aatcaaaaga aaaattctcc caagaattag aaatgagtag
4921 atcaaattat tcacgaatag aatcaggaaa atcagatcca accataaaaa cactagaaca
4981 aattgcaaag ttaactaact caacgctagt agtggattta atcccaaatg agccaacaga
5041 accagaacca gaaacagaat cagaacaagt aacattggat ttagaaatgg aagaagaaaa
5101 aagcaatgac ttcgtgtgaa taatgcacga aatcgttgct tatttttttt aaaagcggta
5161 tactagatat aacgaaacaa cgaactgaat agaaacgaaa aaagagccat gacacattta
5221 taaaatgttt gacgacattt tataaatgca tagcccgata agattgccaa accaacgctt
5281 atcagttagt cagatgaact cttccctcgt aagaagttat ttaattaact ttgtttgaag
5341 acggtatata accgtactat cattatatag ggaaatcaga gagttttcaa gtatctaagc
5401 tactgaattt aagaattgtt aagcaatcaa tcggaaatcg tttgattgct ttttttgtat
5461 tcatttatag aaggtggagt ttgtatgaat catgatgaat gtaaaactta tataaaaaat
5521 agtttattgg agataagaaa attagcaaat atctatacac tagaaacgtt taagaaagag
5581 ttagaaaaga gaaatatcta cttagaaaca aaatcagata agtatttttc ttcggagggg
5641 gaagattata tatataagtt aatagaaaat aacaaaataa tttattcgat tagtggaaaa
5701 aaattgactt ataaaggaaa aaaatctttt tcaaaacatg caatattgaa acagttgaat
5761 gaaaaagcaa accaagttaa ttaaacaacc tattttatag gatttatagg aaaggagaac
5821 agctgaatga atatcccttt tgttgtagaa actgtgcttc atgacggctt gttaaagtac
5881 aaatttaaaa atagtaaaat tcgctcaatc actaccaagc caggtaaaag caaaggggct
5941 atttttgcgt atcgctcaaa atcaagcatg attggcggtc gtggtgttgt tctgacttcc
6001 gaggaagcga ttcaagaaaa tcaagataca tttacacatt ggacacccaa cgtttatcgt
6061 tatggaacgt atgcagacga aaaccgttca tacacgaaag gacattctga aaacaattta
6121 agacaaatca ataccttctt tattgatttt gatattcaca cggcaaaaga aactatttca
6181 gcaagcgata ttttaacaac cgctattgat ttaggtttta tgcctactat gattatcaaa
6241 tctgataaag gttatcaagc atattttgtt ttagaaacgc cagtctatgt gacttcaaaa
6301 tcagaattta aatctgtcaa agcagccaaa ataatttcgc aaaatatccg agaatatttt
6361 ggaaagtctt tgccagttga tctaacgtgt aatcattttg gtattgctcg cataccaaga
6421 acggacaatg tagaattttt tgatcctaat taccgttatt ctttcaaaga atggcaagat
6481 tggtctttca aacaaacaga taataagggc tttactcgtt caagtctaac ggttttaagc
6541 ggtacagaag gcaaaaaaca agtagatgaa ccctggttta atctcttatt gcacgaaacg
6601 aaattttcag gagaaaaggg tttaataggg cgtaataacg tcatgtttac cctctcttta
6661 gcctacttta gttcaggcta ttcaatcgaa acgtgcgaat ataatatgtt tgagtttaat
6721 aatcgattag atcaaccctt agaagaaaaa gaagtaatca aaattgttag aagtgcctat
6781 tcagaaaact atcaaggggc taatagggaa tacattacca ttctttgcaa agcttgggta
6841 tcaagtgatt taaccagtaa agatttattt gtccgtcaag ggtggtttaa attcaagaaa
6901 aaaagaagcg aacgtcaacg tgttcatttg tcagaatgga aagaagattt aatggcttat
6961 attagcgaaa aaagcgatgt atacaagcct tatttagtga cgaccaaaaa agagattaga
7021 gaagtgctag gcattcctga acggacatta gataaattgc tgaaggtact gaaggcgaat
7081 caggaaattt tctttaagat taaaccagga agaaatggtg gcattcaact tgctagtgtt
7141 aaatcattgt tgctatcgat cattaaagta aaaaaagaag aaaaagaaag ctatataaag
7201 gcgctgacaa attcttttga cttagagcat acattcattc aagagacttt aaacaagcta
7261 gcagaacgcc ctaaaacgga cacacaactc gatttgttta gctatgatac aggctgaaaa
7321 taaaacccgc actatgccat tacatttata tctatgatac gtgtttgttt tttctttgct
7381 gtttagcgaa tgattagcag aaatatacag agtaagattt taattaatta ttagggggag
7441 aaggagagag tagcccgaaa acttttagtt ggcttggact gaacgaagtg agggaaaggc
7501 tactaaaacg tcgaggggca gtgagagcga agcgaacact tgatttttta attttctatc
7561 ttttataggt cattagagta tacttatttg tcctataaac tatttagcag cataatagat
7621 ttattgaata ggtcatttaa gttgagcata ttagaggagg aaaatcttgg agaaatattt
7681 gaagaacccg attacatgga ttggattagt tcttgtggtt acgtggtttt taactaaaag
7741 tagtgaattt ttgatttttg gtgtgtgtgt cttgttgtta gtatttgcta gtcaaagtga
7801 ttaaatagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
7861 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
7921 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
7981 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
8041 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
8101 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
8161 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
8221 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
8281 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
8341 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
8401 g