pShuttle-IRES-hrGFP-2
- 产品货号GS-1496
- 销售价格¥ 1020
- 包装规格2μg质粒或0.5ml甘油菌
- 表达类型低
- 载体抗性Kanamycin (卡那霉素)
- 载体大小8900 bp
- 启动子CMV
- 5'测序引物pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC
- 备注说明载体内包含有IRES序列和hrGFP序列
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG? epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).