443143416pGAD424
- 产品货号GS-1644
- 销售价格￥ 680
- 包装规格2μg质粒或0.5ml甘油菌
- 载体抗性Ampicillin (氨苄青霉素)
- 载体大小6659 bp
- 启动子ADH1
pGAD424 generates a hybrid protein that contains the sequences for the GAL4 activation domain (AD; a.a. 768–881) (1). pGAD424 has unique restriction sites located in the MCS region at the 3'- end of the open reading frame for the GAL4 AD sequence. For the construction of a hybrid protein, the gene encoding the protein of interest (or a collection of cDNAs) is ligated into the MCS in the correct orientation and in the correct reading frame such that a fusion protein is generated. The fusion protein is expressed at high levels in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences that have been added to the AD sequence from a heterologous source (2). pGAD424 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae. It carries the bla gene (for ampicillin resistance in E. coli) and the LEU2 nutritional marker that allow yeast auxotrophs carrying pGAD424 to grow on limiting synthetic medium lacking Leu.
0001 GCTTGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC
0061 ATATCCGCAA TGACAAAAAA AATGATGGAA GACACTAAAG GAAAAAATTA ACGACAAAGA
0121 CAGCACCAAC AGATGTCGTT GTTCCAGAGC TGATGAGGGG TATCTTCGAA CACACGAAAC
0181 TTTTTCCTTC CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT
0241 TCCAGTTACT TGAATTTGAA ATAAAAAAAG TTTGCCGCTT TGCTATCAAG TATAAATAGA
0301 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT
0361 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA
0421 GATGGATAAA GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG
0481 TACCGCCGCC AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT
0541 CACTAACAGT AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC
0601 ACAACCAATT GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG
0661 TAAAATTGAT GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC
0721 GTATAACGCG TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA
0781 TAACTATCTA TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCGAATTCCC
0841 GGGGATCCGT CGACCTGCAG AGATCTATGA ATCGTAGATA CTGAAAAACC CCGCAAGTTC
0901 ACTTCAACTG TGCATCGTGC ACCATCTCAA TTTCTTTCAT TTATACATCG TTTTGCCTTC
0961 TTTTATGTAA CTATACTCCT CTAAGTTTCA ATCTTGGCCA TGTAACCTCT GATCTATAGA
1021 ATTTTTTAAA TGACTAGAAT TAATGCCCAT CTTTTTTTTG GACCTAAATT CTTCATGAAA
1081 ATATATTACG AGGGCTTATT CAGAAGCTTT GGACTTCTTC GCCAGAGGTT TGGTCAAGTC
1141 TCCAATCAAG GTTGTCGGCT TGTCTACCTT GCCAGAAATT TACGAAAAGA TGGAAAAGGG
1201 TCAAATCGTT GGTAGATACG TTGTTGACAC TTCTAAATAA GCGAATTTCT TATGATTTAT
1261 GATTTTTATT ATTAAATAAG TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC
1321 TTAGGTTTTA AAACGAAAAT TCTTGTTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT
1381 TTCTCAGGTA TAGCATGAGG TCGCTCTTAT TGACCACACC TCTACCGGCA TGCCCGAAAT
1441 TCCCCTACCC TATGAACATA TTCCATTTTG TAATTTCGTG TCGTTTCTAT TATGAATTTC
1501 ATTTATAAAG TTTATGTACA AATATCATAA AAAAAGAGAA TCTTTTTAAG CAAGGATTTT
1561 CTTAACTTCT TCGGCGACAG CATCACCGAC TTCGGTGGTA CTGTTGGAAC CACCTAAATC
1621 ACCAGTTCTG ATACCTGCAT CCAAAACCTT TTTAACTGCA TCTTCAATGG CCTTACCTTC
1681 TTCAGGCAAG TTCAATGACA ATTTCAACAT CATTGCAGCA GACAAGATAG TGGCGATAGG
1741 GTCAACCTTA TTCTTTGGCA AATCTGGAGC AGAACCGTGG CATGGTTCGT ACAAACCAAA
1801 TGCGGTGTTC TTGTCTGGCA AAGAGGCCAA GGACGCAGAT GGCAACAAAC CCAAGGAACC
1861 TGGGATAACG GAGGCTTCAT CGGAGATGAT ATCACCAAAC ATGTTGCTGG TGATTATAAT
1921 ACCATTTAGG TGGGTTGGGT TCTTAACTAG GATCATGGCG GCAGAATCAA TCAATTGATG
1981 TTGAACCTTC AATGTAGGAA ATTCGTTCTT GATGGTTTCC TCCACAGTTT TTCTCCATAA
2041 TCTTGAAGAG GCCAAAACAT TAGCTTTATC CAAGGACCAA ATAGGCAATG GTGGCTCATG
2101 TTGTAGGGCC ATGAAAGCGG CCATTCTTGT GATTCTTTGC ACTTCTGGAA CGGTGTATTG
2161 TTCACTATCC CAAGCGACAC CATCACCATC GTCTTCCTTT CTCTTACCAA AGTAAATACC
2221 TCCCACTAAT TCTCTGACAA CAACGAAGTC AGTACCTTTA GCAAATTGTG GCTTGATTGG
2281 AGATAAGTCT AAAAGAGAGT CGGATGCAAA GTTACATGGT CTTAAGTTGG CGTACAATTG
2341 AAGTTCTTTA CGGATTTTTA GTAAACCTTG TTCAGGTCTA ACACTACCTG TACCCCATTT
2401 AGGACCACCC ACAGCACCTA ACAAAACGGC ATCAACCTTC TTGGAGGCTT CCAGCGCCTC
2461 ATCTGGAAGT GGGACACCTG TAGCATCGAT AGCAGCACCA CCAATTAAAT GATTTTCGAA
2521 ATCGAACTTG ACATTGGAAC GAACATCAGA AATAGCTTTA AGAACCTTAA TGGCTTCGGC
2581 TGTGATTTCT TGACCAACGT GGTCACCTGG CAAAACGACG ATCTTCTTAG GGGCAGACAT
2641 TAGAATGGTA TATCCTTGAA ATATATATAT ATATTGCTGA AATGTAAAAG GTAAGAAAAG
2701 TTAGAAAGTA AGACGATTGC TAACCACCTA TTGGAAAAAA CAATAGGTCC TTAAATAATA
2761 TTGTCAACTT CAAGTATTGT GATGCAAGCA TTTAGTCATG AACGCTTCTC TATTCTATAT
2821 GAAAAGCCGG TTCCGGCCTC TCACCTTTCC TTTTTCTCCC AATTTTTCAG TTGAAAAAGG
2881 TATATGCGTC AGGCGACCTC TGAAATTAAC AAAAAATTTC CAGTCATCGA ATTTGATTCT
2941 GTGCGATAGC GCCCCTGTGT GTTCTCGTTA TGTTGAGGAA AAAAATAATG GTTGCTAAGA
3001 GATTCGAACT CTTGCATCTT ACGATACCTG AGTATTCCCA CAGTTGGGGA TCTCGACTCT
3061 AGCTAGAGGA TCAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
3121 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA
3181 TGAGTGAGGT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
3241 CTGTCGTGCC AGCTGGATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
3301 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
3361 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
3421 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
3481 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
3541 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
3601 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
3661 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
3721 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
3781 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
3841 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
3901 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
3961 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
4021 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
4081 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
4141 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
4201 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
4261 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
4321 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
4381 ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
4441 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
4501 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
4561 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
4621 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
4681 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
4741 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
4801 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
4861 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
4921 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
4981 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
5041 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
5101 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
5161 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT
5221 CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA
5281 AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC
5341 TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA
5401 CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG
5461 GCATCAGAGC AGATTGTACT GAGAGTGCAC CATAACGCAT TTAAGCATAA ACACGCACTA
5521 TGCCGTTCTT CTCATGTATA TATATATACA GGCAACACGC AGATATAGGT GCGACGTGAA
5581 CAGTGAGCTG TATGTGCGCA GCTCGCGTTG CATTTTCGGA AGCGCTCGTT TTCGGAAACG
5641 CTTTGAAGTT CCTATTCCGA AGTTCCTATT CTCTAGCTAG AAAGTATAGG AACTTCAGAG
5701 CGCTTTTGAA AACCAAAAGC GCTCTGAAGA CGCACTTTCA AAAAACCAAA AACGCACCGG
5761 ACTGTAACGA GCTACTAAAA TATTGCGAAT ACCGCTTCCA CAAACATTGC TCAAAAGTAT
5821 CTCTTTGCTA TATATCTCTG TGCTATATCC CTATATAACC TACCCATCCA CCTTTCGCTC
5881 CTTGAACTTG CATCTAAACT CGACCTCTAC ATTTTTTATG TTTATCTCTA GTATTACTCT
5941 TTAGACAAAA AAATTGTAGT AAGAACTATT CATAGAGTGA ATCGAAAACA ATACGAAAAT
6001 GTAAACATTT CCTATACGTA GTATATAGAG ACAAAATAGA AGAAACCGTT CATAATTTTC
6061 TGACCAATGA AGAATCATCA ACGCTATCAC TTTCTGTTCA CAAAGTATGC GCAATCCACA
6121 TCGGTATAGA ATATAATCGG GGATGCCTTT ATCTTGAAAA AATGCACCCG CAGCTTCGCT
6181 AGTAATCAGT AAACGCGGGA AGTGGAGTCA GGCTTTTTTT ATGGAAGAGA AAATAGACAC
6241 CAAAGTAGCC TTCTTCTAAC CTTAACGGAC CTACAGTGCA AAAAGTTATC AAGAGACTGC
6301 ATTATAGAGC GCACAAAGGA GAAAAAAAGT AATCTAAGAT GCTTTGTTAG AAAAATAGCG
6361 CTCTCGGGAT GCATTTTTGT AGAACAAAAA AGAAGTATAG ATTCTTTGTT GGTAAAATAG
6421 CGCTCTCGCG TTGCATTTCT GTTCTGTAAA AATGCAGCTC AGATTCTTTG TTTGAAAAAT
6481 TAGCGCTCTC GCGTTGCATT TTTGTTTTAC AAAAATGAAG CACAGATTCT TCGTTGGTAA
6541 AATAGCGCTT TCGCGTTGCA TTTCTGTTCT GTAAAAATGC AGCTCAGATT CTTTGTTTGA
6601 AAAATTAGCG CTCTCGCGTT GCATTTTTGT TCTACAAAAT GAAGCACAGA TGCTTCGTT