Gateway? Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway? Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR?/Zeo vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.