The pGEM ? -3Zf(+) Vector is a derivative of the pGEM ? -3Z Vector and contains the origin of replication of the filamentous phage f1. The plasmid serves as a standard cloning vector, as a template for in vitro transcription and as a template for the production of circular ssDNA.
The pGEM ? -3Zf(+) Vector contains SP6 and T7 RNA polymerase promoters flanking a multiple cloning region within the α -peptide coding region of β -galactosidase (1). Insertional inactivation of the α -peptide allows recombinant clones to be directly identified by color screening on indicator plates. The multiple cloning region is unique and includes restriction sites for EcoRI, SacI, KpnI, AvaI, SmaI, BamHI, XbaI, SalI, AccI, HincII, PstI, SphI and HindIII.