Base pairs 5653 AP1 response element 26-67 Minimal TA promoter (pTA) 90-112 luc2 reporter gene 154-1806 SV40 late poly(A) signal 1841-2062 SV40 early enhancer/promoter 2110-2528 Synthetic neomycin phosphotransferase (Neor) coding region 2553-3347 Synthetic poly(A) signal 3372-3420 Reporter Vector primer 4 (RVprimer4) binding region 3487-3506 ColE1-derived plasmid replication origin 3744 Synthetic Beta-lactamase (Ampr) coding region 4535-5395 Synthetic poly(A) signal/transcriptional pause site 5500-5653 Reporter Vector primer 3 (RVprimer3) binding region 5602-5621