载体基本信息

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载体说明

Features of the pGIPZ? lentiviral vector (Figure 2-3, Table 1) that make it a versatile tool for RNAi studies include:

• Ability to perform transfections or transductions using the replication incompetent lentivirus (Shimada, et al. 1995)
• TurboGFP and shRNAmir are part of a bicistronic transcript allowing the visual marking of shRNAmir expressing cells
• Amenable to in vitro and in vivo applications
• Puromycin drug resistance marker for selecting stable cell lines
• Molecular barcodes enable multiplexed screening in pools

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