
This plasmid contains the coding sequence of a dimeric vesion of the fluorescent protein “Keima-Red,” which was cloned from the stony coral whose Japanese name is“Komon-Sango.” CoralHue? dKeima-Red absorbs light maximally at 440 nm and emits red light at 616 nm. Thus CoralHue? dKeima-Red exhibits an extremely large Stokes shift (176 nm). The red fluorescence is stable under usual aerobic conditions. The combination of red emission, a very large Stokes shift, stability at 37℃ in eukaryotic cells, and being dimeric make dKeima-Red a superb reporter protein for labeling proteins or subcellular structures in multicolor fluorescence analyses.