The pcDNA? vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway? pcDNA?-DEST40 vector offers the following key features:
C-terminal V5-6x His tag for easy detection and rapid purification
Cytomegalovirus (CMV) promoter for high-level expression
attR sites for Gateway? cloning, enabling recombination with attL-flanked fragments
Neomycin resistance gene for stable selection
Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
