The pFLAG-CMV?-3 Expression Vector is a 6.3 kb vector used for transient or stable expression in mammalian cells. The vector is a derivative of pCMV5 used for transient or stable expression and secretion of a properly inserted open reading frame as an N-terminal FLAG fusion protein.
The pFLAG-CMV-3 Expression Vector is a shuttle vector containing both bacterial and SV40 origins of replication for propagation in both E. coli and mammalian cells. Efficiency of replication and genomic integration is optimal when using host cells that express the SV40 large T antigen.
The pFLAG-CMV-3-BAP Control Plasmid is a 7.7 kb derivative of pCMV51 used for transient expression and secretion of N-terminal FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The FLAG? epitope is a small, hydrophilic 8 amino acid tag (DYKDDDDK) that provides for sensitive detection and high quality purification using ANTI-FLAG products available from Sigma-Aldrich.