pAmpho Vector expresses the 4070A amphotropic envelope protein under the control of the CMV immediate-early promoter (1). The amphotropic envelope protein can serve as a surrogate viral envelope protein. It interacts on the host cell surface with the Ram-1 protein which acts as a viral receptor for infection (2). pAmpho includes IVS, a synthetic intron known to enhance the stability of the mRNA (3), the pUC origin of replication, and the bacterial ampicillin resistance (Ampr) gene for propagation and antibiotic selection in bacteria.