The pAd?PL-DEST? Gateway? Vector Kit contains the Gateway?-adapted ViraPower? adenoviral expression vector, pAd?PL-DEST? vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.
Advantages
? High efficiency and rapid recombination cloning
? Produces high titer adenoviral stocks
? Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
? Allows gene of interest to be controlled by a promoter of choice
? Produces replication-incompetent virus for enhanced biosafety of the system
? Amenable for use in high-throughput applications
Key Features
? Gateway? Technology for efficient and rapid cloning
? Promoterless vector that allows gene of interest to be controlled by a promoter of choice?
? Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
? Ampicillin selection marker