p10A1 expresses the 10A1 envelope protein under the control of the CMV immediate-early promoter (1).Virus packagedwith the 10A1 envelope protein can enter cells via two different surface molecules, the amphotropic retrovirus receptor or the GALV receptor, and therefore exhibits a broader host range than virus packaged with other envelope proteins (2, 3, 4). p10A1 includes IVS, a synthetic intron known to enhance the stability of the mRNA (5), the pUC origin of replication, and a bacterial ampicillin resistance (Ampr) gene for propagation and antibiotic selection in bacteria.