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pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
• Neomycin resistance gene for selection of stable cell lines
• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.

This pcDNA?3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin? selectable marker and a forward-orientation multiple cloning site.

The pcDNA?3.1 Expression Vector Family

Three untagged versions of pcDNA?3.1 (available separately), each with a different selectable marker (Geneticin?, Zeocin?, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features:
? Cytomegalovirus (CMV) enhancer-promoter for high-level expression
? Large multiple cloning site in either forward (+) or reverse (-) orientations
? Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
? SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,?COS-1 and COS-7)
? Ampicillin resistance gene and pUC origin for selection and maintenance in?E. coli

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